Histological Effect of Saccharomyces Cerevisiae in the Ration on Duodenum of Lambs

Abstract

In this study, sixteen male Awassi lambs were allocated into four experimental groups. The control group (T1) received no yeast, while the treatment groups (T2, T3, and T4) were administered 3g, 5g, and 7g of Saccharomyces cerevisiae (yeast) per lamb, respectively. All lambs were fed a diet consisting of barley grain, yellow corn, soybean meal, salt, and a mixture of vitamins and minerals, with the yeast supplement added accordingly. The feeding trial lasted for 75 days, after which all lambs were slaughtered, and tissue samples were collected from the first, second, and third sections of the duodenum. These samples were fixed in 10% formalin and processed using standard histological techniques to create slides stained with Hematoxylin and Eosin. The slides were then photographed using a digital camera mounted on an Olympus microscope. The findings revealed that groups T1, T2, and T3 exhibited longer villi lined with simple columnar epithelium, with goblet cells present between the epithelial cells. The lamina propria contained numerous mucus glands, along with lymphocytic infiltration and plasma cells. In contrast, group T4 showed necrotic cells from the simple columnar epithelium sloughing off into the duodenal lumen, while other components of the duodenum remained intact.